From Jacksonville Area:

Take I-10 West for 18.4 miles
Take Exit # 343 to merge onto US-301 South for 36.9 miles
Slight right at Kennard St/SR-24 West
Continue to follow SR-24 West for 13.2 miles
Turn right at SR-20/SR-24/SR-26
Continue to follow SR-24/SR-26
Turn left at SR-20 E/SR-329 South
Continue to follow SR-329
Turn right at SW 2nd Ave

Note: Some Towns on US 301 vigorously enforce speeding laws and do speed traps 24/7. Hampton, Lawtey and Waldo have been designated speed traps by Triple A. Adjust your speed accordingly when driving through these areas. Do not exceed posted speed limits!

From Orlando Area:

Start off taking Florida Turnpike North [Portions Toll]
Take the exit onto I-75 North for 56.9 miles
Take Exit # 384 toward Gainesville
Merge onto SW Archer Rd/SR-24 East for 3.2 miles
Slight left at SR-25 N/US-441 N
Turn right at SW 2nd Ave

Area Map [Click To Enlarge]

Directions From Tallahassee Area:

Take I-10 East for 96.7 miles
Take Exit # 296A to merge onto I-75 South for 47.6 miles
Take Exit # 387 for SR-26 toward Gainesville/Newberry
Turn right at W Newberry Rd/SR-26 East
Continue to follow SR-26 East for 5.6 miles
Turn right at SW 3rd St
Turn left at SW 2nd Ave

From Tampa/St. Petersburg Area:

Take I-275 North
Take the exit and merge onto I-75 North for 111 miles
Take Exit # 384 toward Gainesville
Merge onto SW Archer Rd/SR-24 East for 3.2 miles
Slight left at SR-25 N/US-441 N
Turn right at SW 2nd Ave